Basic Plant Genetic Engineering – Principles and Methods, Tissue culture, Direct/Indirect Organogenesis, Marker free technology, Agrobacterium and genegun mediated transformation.
Course Description
Unlock the fundamentals of modern plant biotechnology and genetic engineering through this comprehensive beginner-to-intermediate level course. This course is designed for students, researchers, teachers, and biotechnology enthusiasts who want to understand the principles and applications of plant tissue culture, transgenic technology, and genome editing.
The course begins with the basics of plant tissue culture, including culture media, aseptic techniques, and the role of plant growth regulatory hormones in morphogenesis. Learners will explore direct and indirect organogenesis, selectable and screenable markers, and the structure and function of binary vectors used in plant transformation.
Advanced modules introduce strategies for developing marker-free transgenic plants and provide simplified explanations of CRISPR-Cas genome editing mechanisms. The course also explains how Agrobacterium transfers DNA into plant cells and the principles of gene gun-mediated transformation of plant tissues.
With clear explanations, diagrams, animations, and conceptual discussions, this course makes complex biotechnology topics easy to understand and applicable for academic learning, competitive exams, and research preparation.
The topics addressed in the course are
1. Plant tissue culture
2. Plant Hormones
3. Direct and indirect organogenesis
4. Selectable markers
5. Binary vectors
6. Marker-free transgenic technology
7. CRISPR Cas9 mediated gene editing
8. Agrobacterium mediated plant transformation
9. Gene gun mediated plant transformation
